Changes in Protein Phosphorylation during Salivary Gland Degeneration in Haemaphysalis longicornis

Identification of genes encoding cement-like antigens expressed in the salivary glands of Haemaphysalis longicornis.

A cDNA expression library from the salivary glands of hard tick, Haemaphysalis longicornis, was constructed. Immunoscreening was performed using sera of the rabbit repeatedly infested with ticks and seventeen positive clones were obtained. A BLASTP search suggested that 8 sequences matched with that of hypothetical H. longicornis sequence and one clone encoded HL35 antigen U from the same tick ...

Role of protein phosphorylation in rat salivary gland exocytosis.

Molecular characterization of a Haemaphysalis longicornis tick salivary gland-associated 29-kilodalton protein and its effect as a vaccine against tick infestation in rabbits.

The use of tick vaccines in mammalian hosts has been shown to be the most promising alternative tick control method to current use of acaricides, which suffers from a number of limitations. However, the success of this method is dependent on the identification, cloning, and in vitro expression of tick molecules involved in the mediation of key physiological roles with respect to the biological ...

Interaction of lithium and gentamicin in salivary gland by electron microscopy and protein and DNA measurement

  The effects of gentamicin, lithium and combination of these drugs on ultrastructural morphology of rat submandibular glands were studied. Daily intraperitoneal injection of 40 mg/kg of gentamicin which causes chronic salivary gland toxicity, led to a marked decrease in gland's dry weight and total protein concentration but did not affect DNA content. Electron micrograph (EM) of submandibular ...

Epidemiology of toxoplasmosis: role of the tick Haemaphysalis longicornis

BACKGROUND Toxoplasma gondii infection is mainly caused by ingestion of water or food that is contaminated with oocysts excreted by cats, or by eating raw meat containing T. gondii tissue cysts. However, oral transmission does not explain the common occurrence of toxoplasmosis in a variety of hosts, such as herbivorous animals, birds, and wild rodents. Little information exists on the maintenan...